多糖-硒化多糖复方抗氧化活性的比较

doi:10.11843/j.issn.0366-6964.2017.02.020

Abstract

Abstract:

In order to develop the antioxidant polysaccharide preparations, based on our previous researches, 18 compounds composed with Lycium barbarum polysaccharide (LBP), Atractylodes macrocephala polysaccharide (AMP), Chinese angelica polysaccharide (CAP), Codonopsis pilosula polysaccharide (CPP), Garlic polysaccharide (GP), Lily polysaccharide (LP), Schisandra chinensis polysaccharide (SCP), selenizing LBP (sLBP), selenizing AMP (sAMP), and selenizing CAP (sCAP) with stronger antioxidant activities according to carbohydrate content at 9:1 ratio of polysaccharide to selenizing polysaccharide were chose. Their antioxidant activities were compared. In vitro tests, the scavenging abilities of 18 compounds were compared for 3 kinds of free radicals. In vivo tests, the chicks were injected respectively with 3 compounds each at high and low dosage when they were vaccinated with Newcastle disease vaccine, and the dynamic changes of serum contents of 3 antioxidases and MDA were determined. The results of in vitro tests showed that 18 compounds each at 5 concentrations had different extent scavenging abilities for DPPH radical, hydroxyl radical and ABTS radicals, and the abilities of LBP-sAMP, CAP-sLBP, and CAP-sAMP were stronger. The results of in vivo test showed that in 6 compound groups at 4 time points, the activities of CAT, SOD and GSH-Px were higher or significantly higher than those in control group, and MDA contents were lower or significantly lower than those in control group. In CAP-sLBP group, 3 antioxidases activities were the highest and MDA contents were the lowest. These results indicate that 18 compounds possess differen extent antioxidant activities, the activity of CAP-sLBP is the strongest among 18 compounds and can be considered as the candidate of the antioxidant polysaccharide preparation.

CLC Number:

S853.74

TIAN Wei-jun, LIU Kuan-hui, GAO Zhen-zhen, HOU Ran-ran, YUE Chan-juan, QIN Jia-ling, WANG De-yun, HU Yuan-liang. The Antioxidant Activity Comparison of Polysaccharide-selenizing Polysaccharide Compounds[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 359-368.

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The Antioxidant Activity Comparison of Polysaccharide-selenizing Polysaccharide Compounds

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